Specific receptors

Investigation regarding production of specific bioreceptors with tailored features is essential, since these biomolecules are responsible for the specific signal obtained in the diagnostic tools developed. Therefore, availability of specific receptors is crucial for the general objective of the research at the Nb4D. For this purpose a deep knowledge of the properties of the target are required. Nowadays molecular modeling tools and theoretical calculations are employed to assist in the design of the most appropriate immunogen. This is particularly useful when non-immunogenic small organic molecules constitute the analytical objective. In such cases, an appropriate design of the chemical structure of the hapten is crucial to accomplish the desired antibody features.

Organic synthesis, molecular modeling tools, theoretical calculations and/or monoclonal antibody screening are between the strategies used to accomplish this aim.

Case Studies

An immunochemical strategy based on peptidoglycan synthetic peptide epitopes to diagnose Staphylococcus aureus infections

Citation: Pastells, C.; Acosta, G.; Pascual, N.; Albericio, F.; Royo, M.; Marco, M. P. Analytica Chimica Acta 2015, 889, 203-211.

MICROPLEX; NanoMeDiag (EUI2008-00175)

Case Studies

Molecular Modeling Assisted Hapten Design To Produce Broad Selectivity Antibodies for Fluoroquinolone Antibiotics

Citation: Pinacho DG, Sánchez-Baeza F, Marco MP (2012). Analytical Chemistry 84 (10):4527-4534. URL.

ARGOS (AGL2002-04635-C04-03); Panoptes (AGL2005-07700-C06-1); ELISHA (NMP2-CT-2003-505485)


Disulfide Symmetric Dimers as Stable Pre-hapten Forms for Bioconjugation: A Strategy to Prepare Immunoreagents for the Detection of Sulfophenyl Carboxylate Residues in Environmental Samples

Citation: Estévez, M.-C.; Galve, R.; Sánchez-Baeza, F.; Marco, M. P.,  Chemistry - A European Journal 2008, 14 (6), 1906-1917. URL
NanoBioMol (NAN2004-09415-C05-02); ELISHA (NMP-A-CT-2003-505485-1); AGL2004-20341-E

Preparation of Antibodies and Development of an Enzyme-Linked Immunosorbent Assay (ELISA) for the Determination of Doxycycline Antibiotic in Milk Samples

Citation: Adrian, J.; Fernández, F.; Sánchez-Baeza, F.; Marco, M. P.,  Journal of Agricultural and Food Chemistry 2012, 60 (15), 3837-3846. URL

DETECTA (AGL2008-05578-C05-01)
www.nb4diagnostics.org - nb4d@iqac.csic.es
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