Publication in Talanta


Apostolou, T.; Pascual, N.; Marco, M. P.; Moschos, A.; Petropoulos, A.; Kaltsas, G.; Kintzios, S., Extraction-less, rapid assay for the direct detection of 2,4,6-trichloroanisole (TCA) in cork samples. Talanta 2014, 125 (0), 336-340. URL

A novel biosensor for the detection of the cork taint 2,4,6-trichloroanisole (TCA) has been developed without the need for extraction. The technique involves the membranes of fibroblast cells being engineered with TCA specific antibodies, which act as biosensing systems when suspended in an alginate gel matrix. In the presence of TCA a change in membrane potential can be determined. By changing the way of inserting the TCA-specic antibodies into the cells as well as the interface between the recording unit and the cellular biorecognition elements, TCA could be detected directly in macerated cork tissue without the need for extraction.

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