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Publication in Colloids and Surfaces B: Biointerfaces

11/01/2016



Sanmartí-Espinal, M.; Galve, R.; Iavicoli, P.; Persuy, M.A.; Pajot-Augy, E.; Marco, M.P.; Samitier, J. Immunochemical strategy for quantification of G-coupled olfactory receptor proteins on natural nanovesicles. Colloids and Surfaces B: Biointerfaces, 2016, Volume 139, pg. 269-276.

A novel immunochemical analytical approach for the quantification of membrane proteins (i.e. G-protein-coupled receptors, GPCR) in nanovesicles was developed. The procedure allowed for direct determination of tagged receptros (i.e. c-myc tag) without any previous protein purification or extraction steps. Based on a microplate ELISA format, this immunochemical method quantifies these tags on proteins embedded in nanovesicles with detectability in the picomolar range using protein bioconjugates as reference standards. The applicability of the method is demonstrated through the quantification of the c-myc olfactory receptor (c-myc-OR1740) in the cell membrane nanovesicles. This opens up the possibility to develop well-characterised drug-screening platforms based on G-coupled proteins embedded on membranes.

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